Department of Mechanical Engineering, University of Texas at Austin
"The nanoIR2-s provides us great flexibility on various kinds of materials in our lab, especially biological materials."
“nanoIR combines the powerful functions of nanoscale IR spectra and chemical imaging based on two complementary techniques—photothemal AFM-IR and scattering SNOM. This provides us great flexibility on various kinds of materials in our lab (especially biological materials). Its user-friendly interface and easy operation accelerates my projects. The reliable local customer technical support ensures its great performance all the time.”
Top left: IR nano-imaging using s-SNOM: the phase contrast between silk and silicon, illustrating the dominant protein structure within the amide I vibration; top middle: spectra of a crystalline silk thin film with embedded amorphous silk nanopatterns of ∼30 nm, characterized by AFM–IR,distinguishing nanoscale structural heterogeneity; top right: AFM–IR spectra of electron-induced structural transitions in silk proteins; table 1: quantification of the silk protein secondary structures. Data courtesy of Tao et. al, DOI: 10.1038/ncomms13079
Intercalated Epitaxial Graphene on SiC Substrate Scattering SNOM absorption image 5um x 5um image
Fellow, National Physical Laboratory, Teddington, UK
“The National Physical Laboratory requires extremely precise and reliable measurements and the nanoIR2-s provided exactly the solution we desired.”
"The National Graphene Metrology Centre at NPL will be using the new nano-IR2s system from Anasys Instruments for both nearfield infrared spectroscopy and imaging. Anasys Instruments have been very responsive to our needs and provided exactly the solution we desired. The instrument itself performed out of the box. This level of quality in the technology we use helps NPL provide the most informative and reliable measurement data to our customers.
Prof. Stuart Lyon
AkzoNobel Professor of Corrosion Control, The University of Manchester
"The nanoIR appears to work like magic! It’s AFM-IR technique provides unrivalled access to nanoscale chemical information of relevance to the surface modification of materials. For the first time we have been able to test decades-old hypotheses in surface engineering, corrosion and coatings science with speed, precision and at unprecedented spatial resolution."
The AFM-IR technique has been used to map localised water uptake under humid conditions for a model epoxy phenolic coating to understand corrosion mechanisms. The water sorption is enhanced around regions containing residual epoxy groups (less cross-linked) as shown by the chemical images collected at absorption bands for the CH stretch, weakly bound water and strongly bound water.
Karl W. and Renate Boer Professor and Founding Chair Materials Science and Engineering, University of Delaware
“At no time in the last 20 years has the future for correlating heterogeneity and chemical structure looked brighter.”
"Using the nanoIR2 from Anasys Instruments over the last 18 months, my colleagues and I at the University of Delaware have been able to revisit many exciting research problems in phase separated polymer thin films and identify the chemical composition and morphology of the domains that result. I personally feel that the support that Anasys has given my research group in overcoming sample preparation challenges and by making their instrumental expertise available to us almost 24/7 has provided the opportunity and capability for us to examine many of the fundamental chemical and structural properties of polymers at the nanoscale. At no time in the last 20 years has the future for correlating sample heterogeneity and chemical structure looked brighter and more promising. "
Dr. Ferenc Borondics
Principal Beamline Scientist at the IR spectromicroscopy beamline, Soleil Synchrotron
"The nanoIR2-s is a perfect tool for a multi-user center with a combination of Soft Matter and Condensed Matter research"
"We chose the nanoIR2-s for the Soleil Synchrotron since it is a perfect tool for a multi-user center like ours where we undertake research into a wide range of materials. The nanoIR2-s uniquely combines the complementary techniques of AFM-IR and s-SNOM. AFM-IR provides true, model-free nanoscale IR spectroscopy and is ideal for research on materials such as life sciences, polymer and organics. Additionally s-SNOM is a complementary technique that provides sub-20nm complex optical property imaging and is most suitable for materials like graphene, 2D materials and photonics.”
Professor Alexandre Dazzi, Dept of Physics, University Paris-Sud, and Dr Ferenc Borondics, Principal Beamline Scientist with the nanoIR2-s nanoscale IR spectroscopy system, installed at the SIMS line at the Soleil Synchrotron, Saint Aubin, France
AFM-infrared chemical maps and spectra of Josephin proteins before incubation at 37deg C. (a) AFM height image. Infrared absorption map at (b) 1,700 cm-1, (amide I), (c) 1,655 cm-1, (amide I), (d) 1,300 cm, -1 (amide III). Scale bar, 2mm. (e) Infrared spectra. (f) Average oligomeric infrared spectrum and secondary-structure deconvolution of amide I band Source: F.S. Ruggeri et al,. DOI: 10.1038/ncomms8831
The nanoIR approach showed that ataxin-3 misfold after it aggregates, not before as would be expected by current views on aggregation. In fact, the aggregation of ataxin-3 seems to begin with the individual protein, and then moves onto the formation of intermediate aggregation forms with the original protein structure rather than a misfolded one.
Prof. Giovanni Dietler
Director, Laboratory of the Physics of living matter, EPFL, Lausanne, Switzerland
“Our studies demonstrate the enormous potential of nanoIR in the area of protein misfolding and aggregation.”
"The nanoIR technique was used in our studies to provide a deeper understanding of protein misfolding and aggregation, and was able to confirm previous theories about protein structure that could not be tested due to the limitations of available techniques. There are significant medical and scientific implications of this finding that could change pharmacological and technological approaches to protein aggregation."
State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences
"The nanoIR with AFM-IR is a handy and powerful tool for studying complex polymeric materials. It provided us rich spectroscopic information with unprecedented spatial resolution, which was vital to the success of this project."
average PE content (wt %)
StdDev (wt %)
(a) AFM height image and (b) AFM-IR map of the methyl symmetric C−H bending at 1378 cm−1. (c) AFM-IR spectra taken at the locations marked in (a) and (b), normalized to the 1378 cm−1 band, indicative of different ethylene contents as shown by the intensity of the 1456cm−1 band.
High resolution AFM-IR images (left) may be used to identify sub-surface features and the composition of polymers localized at the surface interface. Average profiling (right) may be performed as a function of height or IR response down to the nanometer scale. Additionally, this technique allows for a better understanding of the formation of newly reacted species.
Dr. Greg Meyers
R&D Fellow, Dow Chemical
"With AFM-IR we can now 'see' the chemistry in the morphology."
"The AFM-IR solves a longstanding need in polymeric materials development for chemical analysis at the nanoscale. By doing it with an AFM, it simultaneously addresses one of the most important missing capabilities of the scanning probe microscopy platform – lack of chemical specificity, thus enabling the further growth of the AFM technique in new applications and markets. We are now able to ‘see’ the chemistry in the morphology."
"The nanoIR2 is a workhorse tool for us. It has powerful capability providing us multiple high value nanoscale chemical applications in an easy to use system. The increasing demand among our customers led to us acquiring our second nanoIR platform"
"The nanoIR2 is a workhorse tool for us. It has multiple high value applications tied to nanoscale chemical composition ranging from Polymer blends and films to nano-contaminants. Anasys has packaged this powerful capability within an easy to use platform. They also provide us with outstanding support. The wide demand among our customers for this nanoscale IR capability led to us acquiring our second nanoIR platform whose capacity utilization has also outstripped our demand forecast."
A. Kulik, F. S. Ruggeri et al., nanoscale Infrared spectroscopy of LHCII proteins and amyloids, Microscopy and analysis, 2014.
Research Associate, Laboratory of the Physics of Living Matter, EPFL (Retired)
"The nanoIR is the most significant advance in AFM based measurements of the last decade."
"The nanoIR is the most significant advance in AFM based measurements of the last decade. By enabling the AFM to get chemical composition information via nanoscale IR spectroscopy, for the first time, it has solved the 35 year old problem that the AFM is chemically blind. Unlike other approaches like TERS, nanoIR is easy to use and reliable, repeatable and does not rely on a proprietary probe. In our Life Sciences focused research group, we used the nanoIR to get protein secondary structure on single protein fibrils which is a major advance for fields like Alzheimer's, Parkinson's, Huntington's and Ataxia’s research. I expect it to similarly impact profoundly many other fields such as antibiotic-resistant bacterial strains, or chromosome studies."
Prof. Wojtek Chrzanowski
Faculty of Pharmacy, University of Sydney
"Breakthrough information from the nanoIR has opened tremendous insights for us into nanotoxicity research"
"We are very happy with our nanoIR. Its breakthrough and unprecedented nanoscale chemical composition information has opened up tremendous insights for us into our nanotoxicity research. It is a very easy to use tool that a new user can be trained up in a day or two. Besides the nanoscale chemistry information, it also provides nanoscale mechanics via Lorentz Contact Resonance and the nanoTA-2 for nanoscale thermal analysis, both of which are very useful for our research. Additionally, the nanoIR has a very easy to use AFM which I find my students tend to prefer over AFMs from other major AFM manufacturers, even for AFM performance. Hence I have no doubt that the nanoIR from Anasys will enjoy widespread adoption in the Field of Pharmaceutical Science and other Academic/Industrial research areas since they have packaged a breakthrough scientific advance into an easy to use platform while adding in other powerful and complementary techniques. Importantly, technical support offered by Anasys is impeccable!"
This data shows how Lorentz Contact Resonance (LCR), which provides nanoscale mechanical analysis, was used for the first time to investigate the uptake and localization of nanodiamond in cells. The topographical image and correlating map of mechanical properties highlights stiffness variations for individual cells treated with nanodiamond (ND). Individual nanomechanical spectra show the contact resonance of the cantilever which is influenced by the mechanical properties of the sample.
Project Leader Global Advanced Characterization, ExxonMobil Chemical (Retired)
"Despite no prior AFM experience, we were able to get AFM-IR up and running and to obtain key insights into our multi-layer film samples within a couple of weeks."
"After evaluating the competing technologies for nanoscale chemical composition of polymers, we chose to bring the powerful nanoscale IR spectroscopy (AFM-IR) technology into ExxonMobil. The AFM-IR instrument is easy to use – we were able to get it up and running quickly and to obtain key insights into our samples within a couple of weeks, despite having no prior AFM experience. We liked that nanoIR spectra correlate well to FTIR spectral libraries without peak shifts or distortions that are intrinsic to techniques that measure scattered light."
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