Applications brief: Preparation of paclitaxel/chitosan co-assembled core-shell nanofibers for drug-eluting stent

Key results


  • nanoIR spectroscopy was used to characterize the core and shell of PTX/CS co-assembled nanofibers
  • Analysis proved the co-assemblec nanofiber core-shell structure consists of a PTX core and CS shell
  • This study provides a convenient method to prepare high PTX loaded NFs, a promising nano-drug carrier used for drug eluding stent

Key words

co-assembly | paclitaxel | chitosan | core-shell nanofibers | drug-eluting stent | nanoIR

Authors

J. Tanga, Y. Liua, B. Zhu, Y. Suc, X Zhuc

Abstract

The paclitaxel/chitosan (PTX/CS) core-shell nanofibers (NFs) are easily prepared by co-assembly of PTX and CS and used in drug-eluting stent. The mixture solution of PTX (dissolved in ethanol) and CS (dissolved in 1% acetic acid water solution) under sonication will make the formation of NFs, in which small molecule PTX co-assembles with biomacromolecular CS through non-covalent interactions. The obtained NFs are tens to hundreds nanometers in diameter and millimeter level in length. Furthermore, the structure of PTX/CS NFs was characterized by confocal laser scanning microscopy (CLSM), zeta potential, X-ray photoelectron spectroscopy (XPS) and nanoscale infra-red (nanoIR), which provided evidences demonstrated that PTX/CS NFs are core-shell structures. The ‘shell’ of CS wrapped outside of the NFs, while PTX is located in the core. Thus it resulted in high drug loading content (>40 wt.%). The well-controlled drug release, low cytotoxicity and good haemocompatibility were also found in drug carrier system of PTX/CS NFs. In addition, the hydrophilic and flexible properties of NFs make them easily coating and filming on stent to prepare drug-eluting stent (DES). Therefore, this study provides a convenient method to prepare high PTX loaded NFs, which is a promising nano-drug carrier used for DES and other biomedical applications. The possible molecular mechanism of PTX and CS co-assembly and core-shell nanofiber formation is also explored.


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Nano-IR characterization of co-assembled PTX/CS (2:2) NFs.
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